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CST Anti-rabbit IgG, HRP-linked Antibody

簡(jiǎn)要描述：CST Anti-rabbit IgG, HRP-linked Antibody 來自CST公司的7074S，HRP標(biāo)記山羊抗兔二抗，可以做WB

  • 產(chǎn)品型號(hào)：7074S
  • 廠商性質(zhì)：代理商
  • 更新時(shí)間：2024-05-07
  • 訪  問  量：2450

詳細(xì)介紹

品牌CST貨號(hào)7074S
規(guī)格1ml供貨周期一周

Product Usage Information

Recommended Antibody Dilutions:

1:1000–1:3000

20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K

SignalFireTM ECL Reagent #6883 1:1K–1:3K

SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K

SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K

Storage

Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.

Product Description

Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection.  This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

    Species Reactivity

    Species reactivity is determined by testing in at least one approved application (e.g., western blot).

    Applications Key

    WB: Western Blotting

    Cross-Reactivity Key

    H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected


    僅限科研使用

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Applications:

    WB

    Source/Isotype:

    Goat 








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